Westase™
Applications:
- Digestion of Yeast Cell Walls
Description:
Westase™ possesses complex lytic activities for yeast cell walls mainly consisting of β-1,6 glucanase and β-1,3 glucanase activity. This enzyme works well for Ascosporogenous yeasts such as Saccharomyces cerevisiae. It can also be used with fission yeasts such as Schizosaccharomyces pombe, which cannot be protoplasted with simple Zymolyase treatment or Basidiosporogenous yeasts,
Manufactured by Ozeki Corporation.
Storage:
4°C (dry condition)
Source:
Streptomyces rochei DB-34. Westase™ is prepared from liquid culture supernatant of Streptomyces rochei DB-34.
Form:
Lyophilized powder (containing celite as the excipient)
Definition of Activity:
β-1,6 glucanase activity: One unit is defined as the amount required to release 1 µmol reducing sugar from 10 mg/mL Pustulan solution in 1 min. at 37°C, pH 6.0.
Lytic activity: One unit is defined as the amount required to cause a 1% decrease in absorbance at 660 nm in 1 min. at 30°C, pH 6.0 when using cell wall fraction of Cryptococcus albidus IFO 0612 as a substrare.
Specific Activity:
| β-1,6 glucanase activity: | ≥ 400 units/g |
| Lytic activity: | > 35,000 units/g |
| DNase activity: | ND |
