M13 mp18 RF DNA
Description:
This DNA is a phage vector suitable for DNA sequencing by dideoxy method. Since it has multi-cloning sites on lacZ' region, any DNA insertion into this phage vector can be easily verified using soft-agar plates containing IPTG and X-Gal. Moreover, the cloned DNA can be directly used for DNA sequencing, because it is a single-strand DNA when isolated from the phage particle. M13 primer is available for DNA sequencing. Using Deletion Kit for Kilo-Sequencing (Cat.# TAK 6030), DNA sequencing of the cloned DNA with kilo-bases can also be performed.
Form:
10 mM Tris-HCl (pH 8.0), 1 mM EDTA
Concentration:
250-1,000 µg/mL
Storage:
–20°C
Notes:
When cloning for a long DNA fragment (e.g. above 4 to 5 kbp) using M13 phage vector, some deletion is often found during the culture.
Purity
- Contains over 70% double-strand covalently closed circular form I (RF I).
- It is confirmed that the multi-cloning site is well-maintained upon sequence analysis by dideoxy method.
- It is confirmed that the vector contains a single cleavage site for EcoR I, Sac I, Kpn I, Sma I, BamH I, Xba I, Sal I, Pst I, Sph I and Hind III upon restriction analysis by the enzymes.
GenBank
- Entry name: M13MP18
- Accession No.: X02513
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