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Home > Products > Cell Biology > Cell Adhesion & Extracellular Matrix > Glycocalicin EIA Kit Details

Glycocalicin EIA Kit

Applications:

  • Quantitative Determination of Human Glycocalicin in Platelet Extract, Plasma and Serum

Description:

Glycocalicin is a 110-135 kDa proteolytic fragment of the platelet glycoprotein Ib α-chain (GPIb), which is presumed to be cleaved by cell surface protease proteolysis, such as calpain1. Glycocalicin is believed to include the binding domain structure of GPIb for ristocetin-dependent von Willebrand factor2,3, thrombin4, and quinidine-dependent antibody receptor5. Recently, it has been shown that Glycocalicin circulated in vivo6 and further studies indicate that plasma levels of Glycocalicin might be a useful marker in the classification of thrombocytopenic disorders7,8. In addition, Glycocalicin levels elevated in the supernatants of platelet concentrates during storage might reflect the shedding of GPIb from stored platelet membranes9,10.

References:

  1. Solum, N.O. et al., (1980) Biochem. Biophys. Acta. 597, 235.
  2. Okumura, T. et al., (1976) Thromb. Res. 8, 701.
  3. Coller, B.S. et al., (1983) Blood 61, 99.
  4. Ganguly, P. et al., (1979) Br. J. Haematol. 42, 137.
  5. Kunicki, T.J. et al., (1978) J. Clin. Invest. 62, 716.
  6. Coller, B.S. et al., (1984) J. Clin. Invest. 73, 794.
  7. Steinberg, M.H. et al., (1987) N. Eng. J. Med. 317, 1037.
  8. Kunishima, S. et al., (1991) Clin. Chem. 37, No.2, 169.
  9. Bessos, H. et al., (1990) Thromb. Res. 59, 497.
  10. Bessos, H. et al., (1993) Transfusion Med. 3, 115.

Kit Components:

Antibody Coated Microtiter plate 1 plate (8 well × 12 strips)
Antibody-POD Conjugate (PL52-4) 1 vial (for 11 mL
Standard 1 vial (640 ng)
Sample Diluent 2 vials (2 x 11 mL)
Substrate Solution (TMBZ) 1 vial (12 mL)

Performance

Range of assay: 10–640 ng/mL
Sensitivity: 10 ng/mL
Assay duration: 2.5 hours for assay protocol
Specificity: This kit specifically measures human Glycocalicin
Test specimen type: Human platelet extract, plasma, serum; If each test sample is run in duplicate, about 210 µL (100 µL per assay well plus approx. 10 µL for waste per transfer) is required.

Storage:

2–8°C

Glycocalicin EIA Kit TAK MK105 96 assays 525.00
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*All prices in USD