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Home > Products > Molecular Biology > Modifying Enzymes: Kinases & RT > Terminal Deoxynucleotidyl Transferase Details

Terminal Deoxynucleotidyl Transferase

Applications:

  • Tailing Reactions to add Complementary Homopolymer Tails to Vectors and cDNA, such as in the Okayama-Berg Method
  • Labeling the 3'-Ends of DNA Fragments using [32P]dNTP or [32P]ddNTP

Description:

Terminal Deoxynucleotidyl Transferase (TdT) does not require a template for activity, and catalyzes the incorporation of deoxynucleotides into the 3'-OH termini of single- or double-stranded DNA. It requires an oligodeoxynucleotide of at least three bases as a primer.

Unit Definition:

One unit is the amount of enzyme that incorporates 1 nmol of [3H]dATP into acid-insoluble products in 1 hour at 37°C and pH 7.2, with calf thymus, DNA that is treated with DNase and heat-denatured (activated calf thymus DNA) as the initiator.

Purity

Endonuclease, Exonuclease and Nickase activity were not detected as judged via gel electrophoresis.

Kit Components:

Supplied Buffer
  • 5X TdT Buffer [500 mM HEPES (pH 7.2), 40 mM MgCl2, 0.5 mM DTT]
  • 0.1% BSA

Form:

60 mM potassium phosphate (pH 7.2), 150 mM KCl, 1 mM 2-mercaptoethanol, 50% glycerol

Source:

E. coli recombinant

Concentration:

7–15 units/µL

Storage:

–20°C

Terminal Deoxynucleotidyl Transferase TAK 2230A 300 units 42.00
Terminal Deoxynucleotidyl Transferase TAK 2230B (A x 5) 1500 units 168.00
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