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Home > Products > Molecular Biology > Modifying Enzymes: Nucleases > Micrococcal Nuclease Details

Micrococcal Nuclease

Applications:

  • Digestion of Nucleotides included in Crude Cell Extract
  • Digestion of Chromatin for Nucleosome Preparation

Description:

Micrococcal Nuclease is an endonuclease that preferentially digests single-stranded DNA or RNA, especially at AT or AU rich region, but will also digest double-stranded DNA or RNA. This enzyme digests 5'-phosphodiester bonds of DNA and RNA, and yields 3'-phosphate mononucleotides and oligonucleotides. This enzyme requires Ca2+ and is completely inactivitated by EDTA or EGTA.

Concentration:

15–30 units/µ/L

Form:

50 mM Tris-HCl (pH 8.0), 10 mM 2-mercaptoethanol, 50 mM NaCl, 50% glycerol

Supplied Buffer

10X Micrococcal Nuclease [200 mM Tris-HCl (pH 8.0), 50 mM NaCl, 25 mM CaCl2]

Source:

Staphylococcus aureus

Unit Definition:

One unit is the amount of enzyme that produces 1 OD260 of acid-soluble products in 30 minutes at 37°C and pH 8.0, with heat-denatured calf thymus DNA as the substrate.

Purity

Nucleasae activity is not detected after incubation of 1 µg λ DNA with 50 units of this enzyme for 10 minutes at 37°C in the reaction enzyme buffer (1 x H buffer), as judged from the agarose gel electrophoresis pattern. This enzyme is more than 95% homogenous as judged by SDS-PAGE.

Storage:

–20°C

Micrococcal Nuclease TAK 2910A 15,000 U 138.00
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