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Home > Products > PCR Products > Real Time (qPCR) Kits > BluePrint™ RT Reagent Kit (for Real Time) Details

BluePrint™ RT Reagent Kit (for Real Time)

Features:

  • Fast and Easy to Use: Optimized for qRT-PCR. Complete RT Reaction in 15 minutes.
  • Superior qPCR Performance: When used with any Real Time PCR Enzyme

Description:

BluePrint™ RT Reagent Kit is designed to perform reverse transcription that has been optimized for real-time Rt-PCR. It uses the new BluePrint™ RTase with excellent elongation ability for quick, efficient cDNA template synthesis for Real Time PCR. The protocol is simple and suitable for high throughput analysis. This kit can be used in combination with qPCR reagents from most major suppliers including Takara's SYBR® Premix Ex Taq™ (Perfect Real Time), SYBR® Premix Ex Taq™ II (Perfect Real Time) or Premix Ex Taq™ (Perfect Real Time) for 2-step Real Time RT-PCR.

Optimized protocols are provided for use with either SYBR® Green or TaqMan® probe assay conditions.

 

 

 

 

Kit Components:

5X BluePrint™ Buffer for Real Time1   400 μL
BluePrint™ Enzyme Mix   100 μL
Oligo dT Primer 50 μM 100 μL
Random 6 mers 100 μM 400 μL
RNase Free dH2O   1 mL X 2
EASY Dilution for Real Time PCR2   1 mL
1: Contains dNTP Mixture and Mg2+.
2: Used as a dilution solution for creating dilution series of total RNA or cDNA. In contrast to dilution with water or TE, EASY Dilution facilitates accurate low concentration dilution. Diluted templates can be used as the template for reverse transcription or PCR reactions, because the EASY Dilution does not inhibit either reverse transcription or PCR enzyme activity. EASY Dilution is also available separately: EASY Dilution (for Real Time PCR) (TAK 9160).
Note: EASY Dilution has been tested with Takara Bio’s real-time PCR reagents. Compatibility with products from other manufacturers has not yet been verified.

BluePrint™ RT Reagent Kit (for Real Time) TAK RR737A 200 reactions 401.00
BluePrint™ RT Reagent Kit TAK RR737B 800 reactions 1372.00
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