BluePrint™ RT PCR Kit
Features:
- Excellent Elongation: able to synthesize long cDNAs (up to 12 kb) with little background.
- Exceptional Efficiency on GC-rich Templates can synthesize templates >70% GC content
- Two Step RT Reaction: separate RT and PCR reactions
Description:
BluePrint™ RT-PCR Kit, a 2-step RT-PCR Kit for endpoint PCR, is designed to provide excellent elongation and highly efficient amplification of cDNA templates. BluePrint™ RTase is a newly developed MMLV (Moloney Murine Leukemia virus)-based reverse transcriptase.
BluePrint™ RTase demonstrates robust and excellent elongation ability and the ability to synthesize cDNA up to 12 kb in length. In addition, it can synthesize cDNA efficiently at standard reverse transcription temperatures (42°C), even when RNA templates contain GC rich regions or complex structures. This avoids high temperature reactions which may cause RNA decomposition.
This kit includes all reagents necessary for the reverse transcription of RNA to cDNA and cDNA amplification using PCR.
Kit Components:
| 1. BluePrint™ RTase (2 step) | 25 µL |
| 2. 5X BluePrint™ RT Buffer | 200 µL |
| 3. Recombinant RNase Inhibitor (40 U/µL) | 25 µL |
| 4. dNTP Mixture (10 mM each) | 150 µL |
| 5. Oligo dT Primer (2.5 µ M) | 50 µL |
| 6. Random 6 mers (20 µ M) | 50 µL |
| 7. TaKaRa Ex Taq™ HS (5 U/µL) | 25 µL |
| 8. 10 X PCR Buffer II | 250 µL |
| 9. Control F-1 Primer2 (20 µM) | 10 µL |
| 10. Control R-1 Primer3 (20 µM) | 10 µL |
| 11. Positive Control RNA (2 × 105 copies/µL) | 20 µL |
| 12. RNase Free dH2O | 1 mL |
| 1. 50 reactions of [Reverse transcription 20 µL → PCR 50 µL] | |
| 2. Upstream sense primer for Positive Control RNA | |
| 3. Downstream anti-sense primer for Positive Control RNA | |
Notes:
Limited Use Label License: [P1][M57][L15]
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