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Home > Products > Molecular Biology > Gene Transfer & Expression > RetroNectin® Reagent and RetroNectin® Precoated Dishes Details

RetroNectin® Reagent and RetroNectin® Precoated Dishes

Applications:

  • High-efficiency retrovirus-mediated gene transfer into target cells

Features:

  •  Highly efficient gene transduction into hematopoietic cells and commonly used cell lines
  • No polybrene required for infection of cells with a virus (polybrene can be toxic to cells)

Description:

Fibronectins (FNS) are multifunctional cell adhesive glycoproteins present in the extracellular matrix and plasma. RetroNectin® (CH-296) is a chimeric peptide of recombinant human fibronectin fragments produced in E. coli, consisting of three functional domains: a central cell-binding domain (type III repeat, 8-10), heparin-binding domain II (type III repeat, 12-14), and a CS1 site within the alternatively-spliced IIICS region. It is a 574 amino acid protein with a molecular weight of 63 kDa.

When coated on the surface of containers such as culture dishes, petri dishes, flasks or bags, RetroNectin® significantly enhances retrovirus-mediated gene transduction into mammalian cells. This enhancement is hypothetically due to co-localization of retroviral particles and target cells on the molecules of RetroNectin®. Virus particles bind RetroNectin® via interaction with heparin-binding domain II, and target cells bind mainly through the interaction of the cell surface integrin receptor VLA-4 with the fibronectin CS1 site. Additionally, cells may also bind through the interaction of another fibronectin ligand (RGDS in repeat 10) within the central cell-binding domain with a corresponding integrin receptor VLA-5 on the cell surface.

Improve Viral Transductions with RetroNectin® Reagent

Recombinant fibronection molecule from Takara Bio colocalizes virus and cells to facilitate gene transfer

    • Improves gene transfer efficiency in hard-to-infect cell types and stem cells
    • Multivalent molecule simultaneously binds virus particles and cell surface proteins, maximizing cell-virus contact
    • Ideal for use with Lenti-X™ and Retro-X™ Expression Systems

 

See the application note for more information.

Form:

Sterilized Solution in 12.5 mM sodium citrate (pH 6.2) and 1.25% sucrose.

Plate Coating

RetroNectin® Reagent is coated onto plates at a concentration of 20–100 μg/mL to cover a plate at 4–20 μg/cm2. RetroNectin® Precoated Dishes are 3.5 cm diameter dishes, precoated at 40 µg/mL, and ready for use.

Examples of Cell Transduction Efficiency with RetroNectin®
Cell
Efficiency of Gene Transfer (%)
TF-1
53.3
HEL
28.1
NIH/3Y3
80.5
CD34+ bMC
86.9
Mice BM MNC fraction
29.2
K-562
93.5
HL-60
89.4

References:

  1. Hanenberg, H. et al. (1996) Nature Medicine 2:876-82.
  2. Kimizuka, F. et al. (1991) J. Biochem. 110:284-91.

 

For research use only. Not for use in diagnostic or therapeutic procedures.

Limited Use Label License: [L9][M62]

Figure Thumbnails:

RetroNectin® Reagent TAK T100A 0.5 mg 166.00
RetroNectin® Reagent TAK T100B 2.5 mg 645.00
RetroNectin® Precoated Dishes TAK T110A 10 precoated dishes 331.00
Product Name Product Number Size Price Qty Add

*All prices in USD