DNA Molecular Weight Standard Markers
Applications:
- Determination of the molecular weight of nucleic acids
Form:
- TAK 3401-3413A: 10 mM Tris-HCl, pH 8.0, 1 mM EDTA
- TAK 3407A/B: 10 mM Tris-HCl, pH 7.4, 1 mM EDTA
- TAK 3415: 10 mM Tris-HCl, pH 8.0, 1 mM EDTA, 6% Glycerol, 0.02% Bromophenol Blue, 0.02% Xylene Cyanol, 0.02% NaN3
Note: Since the terminal fragments of the λ DNA digests contain COS ends, heat treatment is required before use. Denaturation of the fragments may occur unless dilution and heating is performed in TE or TEN buffer. Also, avoid use of these markers on polyacrylamide gels.
Description:
Takara offers a variety of DNA ladders suitable for different applications. Several standard λ and ΦX174 digests are offered, as well as 20 bp–2.5 kb DNA ladders. A Wide Range Ladder, which consists of 16 quantitated fragments from 50–10,000 bp, allows both sizing and quantitation over a broad size range. This ladder is premixed with loading dyes and glycerol for direct gel electrophoresis.
Supplied 6X Loading Buffer: 30% glycerol, 30 mM EDTA, 0.03% Bromophenol Blue, 0.03% Xylene Cylanol




