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DNA Molecular Weight Standard Markers

Applications:

  • Determination of the molecular weight of nucleic acids

Form:

  • TAK 3401-3413A: 10 mM Tris-HCl, pH 8.0, 1 mM EDTA
  • TAK 3407A/B: 10 mM Tris-HCl, pH 7.4, 1 mM EDTA
  • TAK 3415: 10 mM Tris-HCl, pH 8.0, 1 mM EDTA, 6% Glycerol, 0.02% Bromophenol Blue, 0.02% Xylene Cyanol, 0.02% NaN3

Note: Since the terminal fragments of the λ DNA digests contain COS ends, heat treatment is required before use. Denaturation of the fragments may occur unless dilution and heating is performed in TE or TEN buffer. Also, avoid use of these markers on polyacrylamide gels.

Description:

Takara offers a variety of DNA ladders suitable for different applications. Several standard λ and ΦX174 digests are offered, as well as 20 bp–2.5 kb DNA ladders. A Wide Range Ladder, which consists of 16 quantitated fragments from 50–10,000 bp, allows both sizing and quantitation over a broad size range. This ladder is premixed with loading dyes and glycerol for direct gel electrophoresis.

Supplied 6X Loading Buffer: 30% glycerol, 30 mM EDTA, 0.03% Bromophenol Blue, 0.03% Xylene Cylanol

Figure Thumbnails:

20 bp DNA Ladder TAK 3409A 50 μg (100 lanes)
  • 13 fragments
  • 20 to approx. 500 bp
  • 100, 200 and 500 bp reference bands
  • 200 ng/µL 119.00
    20 bp DNA Ladder TAK 3409B 200 reactions (50 µL x 2) 213.00
    20 bp DNA Ladder (Dye Plus) TAK 3420A 100 reactions (500 µL) 119.00
    20 bp DNA Ladder (Dye Plus) TAK 3420B 200 reactions (500 µL x 2) 213.00
    50 bp DNA Ladder (Dye Plus) TAK 3421A 100 reactions (500 µL) 119.00
    50 bp DNA Ladder (Dye Plus) TAK 3421B 200 reactions (500 µL x 2) 213.00
    100 bp DNA Ladder TAK 3407A 50 μg (100 lanes)
  • 11 fragments
  • 100 to approx. 1,500 bp
  • 500 bp reference bands
  • 100 ng/µL 119.00
    100 bp DNA Ladder TAK 3407B 100 μg (200 lanes, A x 2)
  • 11 fragments
  • 100 to approx. 1,500 bp
  • 500 bp reference bands
  • 100 ng/µL 213.00
    100 bp DNA Ladder (Dye Plus) TAK 3422A 100 reactions (500 µL) 119.00
    100 bp DNA Ladder (Dye Plus) TAK 3422B 200 reactions (500 µL x 2) 213.00
    200 bp DNA Ladder TAK 3410A 250 μg (100 lanes)
  • 13 fragments
  • 200 to approx. 4,000 bp
  • 1,000 and 2,000 bp reference bands
  • 200 ng/µL 119.00
    200 bp DNA Ladder TAK 3410B 200 reactions 213.00
    200 bp DNA Ladder (Dye Plus) TAK 3423A 100 reactions (500 µL) 119.00
    200 bp DNA Ladder (Dye Plus) TAK 3423B 200 reactions (500 µL x 2) 213.00
    250 bp DNA Ladder (Dye Plus) TAK 3424A 100 reactions 100.00
    250 bp DNA Ladder (Dye Plus) TAK 3424B 200 reactions 180.00
    500 bp DNA Ladder TAK 3411A 250 μg (100 lanes)
  • 10 fragments
  • 500 to approx. 5,000 bp
  • 2,000 bp reference bands
  • 200 ng/µL 102.00
    500 bp DNA Ladder TAK 3411B 200 reactions 213.00
    500 bp DNA Ladder (Dye Plus) TAK 3425A 100 reactions (500 µL) 100.00
    500 bp DNA Ladder (Dye Plus) TAK 3425B 200 reactions (500 µL x 2) 180.00
    1 kb DNA Ladder TAK 3412A 50 μg (100 lanes)
  • 10 fragments
  • 1 to approx. 10 kb
  • 5,000 bp reference bands
  • 200 ng/µL 100.00
    1 kb DNA Ladder TAK 3412B 200 reactions 213.00
    1 kb DNA Ladder (Dye Plus) TAK 3426A 100 reactions (500 µL) 100.00
    1 kb DNA Ladder (Dye Plus) TAK 3426B 200 reactions (500 µL x 2) 180.00
    2.5 kb DNA Ladder TAK 3413A 50 μg (100 lanes)
  • 14 fragments
  • 2.5 to approx. 35 kb
  • 10 kb reference band
  • 200 ng/µL 119.00
    2.5 kb DNA Ladder TAK 3413B 200 reactions 213.00
    Wide-Range DNA Ladder TAK 3415A 1 mL (100 lanes)
  • 16 quantitated fragments
  • 50 bp to 10 kb
  • 1,400/1,550 bp reference bands
  • 90 ng/µL 119.00
    Wide-Range DNA Ladder TAK 3427A 100 reactions 500 µL
  • 100-2,000 bp
  • 119.00
    Wide-Range DNA Ladder TAK 3427B 200 reactions (500 µL x 2)
  • 100-2,000 bp
  • 213.00
    Wide-Range DNA Ladder TAK 3428A 100 reactions (500 µL)
  • 100-5,000 bp
  • 119.00
    Wide-Range DNA Ladder TAK 3428B 200 reactions (500 µL x 2)
  • 100-5,000 bp
  • 213.00
    φX174 Hae III Digest TAK 3405A 20 μg (100 lanes)
  • 11 fragments
  • 72 to 1,353 bp
  • Use to size fragments <1 kb
  • 200-800 ng/µL 56.00
    φX174 Hae III Digest TAK 3405B 100 μg (500 lanes, A x 5)
  • 11 fragments
  • 72 to 1,353 bp
  • Use to size fragments <1 kb
  • 200-800 ng/µL 221.00
    φX174 Hinc II Digest TAK 3406A 20 μg (100 lanes)
  • 13 fragments
  • 79 to 1,057 bp
  • Use to size fragments <1 kb
  • 200-800 ng/µL 56.00
    φX174 Hinc II Digest TAK 3406B 100 μg (500 lanes, A x 5)
  • 13 fragments
  • 79 to 1,057 bp
  • Use to size fragments <1 kb
  • 200-800 ng/µL 221.00
    pHY Marker TAK 3404A 20 μg (100 lanes)
  • 9 fragments
  • 80 to 4,870 bp
  • 200-800 ng/µL 129.00
    pHY Marker TAK 3404B 100 μg (500 lanes, A x 5)
  • 9 fragments
  • 80 to 4,870 bp
  • 200-800 ng/µL 515.00
    λ-EcoT14 I Digest TAK 3401 100 μg (100 lanes)
  • 11 fragments
  • 74 to 19,329 bp
  • Use to size fragments from 1-8,000 bp
  • 200-800 ng/µL 56.00
    λ-BstP I Digest TAK 3402 100 μg (100 lanes)
  • 14 fragments
  • 117 to 8,453 bp
  • Use to size fragments from 1-5,000 bp
  • 200-800 ng/µL 56.00
    λ-Hind III Digest TAK 3403 100 μg (100 lanes)
  • 8 fragments
  • 125 to 23,130 bp
  • Use to size fragments from 2-10 kb
  • 200-800 ng/µL 56.00
    λ-EcoT14 I/Bgl II Digest TAK 3408 100 μg (100 lanes)
  • 18 fragments
  • 60 to 22,010 bp
  • Use to size fragments from 1-20 kb
  • 200-800 ng/µL 64.00
    Product Name Product Number Size Features Concentration Price Qty Add

    *All prices in USD