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TaKaRa Taq™ and Premix Taq™ (TaKaRa Taq™ Version)
Applications:
- Routine PCR
- Thermal cycle sequencing
Description:
Takara Taq™ is a recombinant, thermostable, 94 kDa DNA polymerase encoded by the DNA polymerase gene of the Thermus aquaticus YT-1 strain which has been cloned into Escherichia coli. It has been shown to have essentially the same characteristics as native Taq DNA polymerase.
TaKaRa Taq™ is a versatile thermostable DNA polymerase suitable for a variety of standard PCR applications. The enzyme is also supplied in a premix format (a mixture of enzyme, buffer, and dNTPs) which simplifies the setup of PCR reactions and minimizes pipetting steps.
PCR Performance
Enzyme performance is confirmed by successful amplification of an 8 kb λ DNA template. Amplification of a single copy gene by PCR is also confirmed using a 2.9 kb human genomic DNA template and human p53-primers.Source:
Recombinant E. coli
Kit Components:
| TAK R001A | |
| TaKaRa Taq™ | 250 U (5 U/μL)* |
| 10X PCR Buffer (contains 15 mM MgCl2) | 1 mL |
| dNTP Mixture (2.5 mM each dNTP) | 800 μL |
| TAK R001AM | |
| TaKaRa Taq™ | 250 U (5 U/μL)* |
| 10X PCR Buffer (without Mg2+) | 1 mL |
| 25 mM MgCl2 | 1 mL |
| dNTP Mixture (2.5 mM each dNTP) | 800 μL |
| TAK R004A - Premix Taq (TaKaRa Taq™ Version) | |
| 2X Taq™ Polymerase Premix | 6 x 500 μL** |
| *Protocol recommends the use of 1.25 U per 50 μL reaction. | |
| **Contains TaKaRa Taq™, PCR Buffer and dNTPs. | |
Storage:
–20°C. Avoid repeated freeze-thaws of TaKaRa Taq™ and dNTPs. Once thawed, aliquot into separate tubes and store at –20°C.
Limited Use Label License: [P1]
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