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Home > Products > Molecular Biology > DNA Labeling & Detection Kits > Ladderman™ DNA Labeling Kit Details

Ladderman™ DNA Labeling Kit

Applications:

  • Random Primer Labeling of DNA Using Thermostable Bca DNA Polymerase

Description:

The Ladderman™ Labeling Kit is a novel random primer labeling system that takes advantage of longer primers and a thermostable DNA polymerase isolated from Bacillus caldotenax YT-G (Bca DNA polymerase). Unlike the E. coli Klenow fragment or modified T7 DNA polymerase, thermostable Bca DNA polymerase allows stable polymerization through templates with high GC content (highly structured regions). The longer 9-mer primers increase the efficiency of incorporation of labeled nucleotides up to 80%.

With this kit, random priming is accomplished within 10 minutes to create efficiently-labeled DNA probes. The kit is designed for use with [32P]-α-, [35S]-α-, or [3H]-α-dCTP labels, but it can also be used with biotin, digoxigenin or DNP-labeled dUTPs by replacement of the dNTP mixture. Generally, a probe with specific activity of >109 dpm/mg DNA will be obtained with [32P]-α-dCTP (approx. 3,000 Ci/mmol, 111 TBq/mmol).

Kit Components:

Random Primer (9-mer) 80 μL
10X Buffer 100 μL
dNTP Mixture 100 μL
(0.2 mM each of dATP, dGTP, and dTTP)
Bca DNA Polymerase (2 U/μL) 40 μL
(cloned and modified B. caldotenax DNA polymerase)
Control DNA solution (25 ng/μL) 10 μL
(λ DNA digested with Hind III, 25 ng/µL)

References:

  1. Feinberg, A.P. and Vogelstein, B. (1984) Anal. Biochem. 137:266-7.
  2. Feinberg, A.P. and Vogelstein, B. (1983) Anal. Biochem. 132:6-13.

 

Limited Use Label License: [M21]

Ladderman™ DNA Labeling Kit TAK 6046 40 reactions 154.00
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*All prices in USD