Takara
PCR How to Select the Best PCR Enzyme Guide to Takara PCR Polymerases High Fidelity PCR High Speed PCR High Performance PCR DNA Bisulphite Modification Real Time (qPCR) Kits General PCR Products RT-PCR and RACE Food Pathogen Detection & Screening Kits PCR Probes & Primer Sets Molecular Biology Cloning, Mapping & cDNA Synthesis Gene Transfer & Expression RNA/Protein/DNA Extraction Nucleic Acids Electrophoresis Instrumentation DNA Labeling & Detection Kits Sequencing, Mutagenesis & Mutation Analysis Molecular Biology Reagents Restriction Enzymes Modifying Enzymes: Kinases & RT Modifying Enzymes: Phosphatases Modifying Enzymes: Ligases Modifying Enzymes: DNA Polymerase Modifying Enzymes: RNA Polymerase Modifying Enzymes: Nucleases Modifying Enzymes: Rnase Inhibitor DNA Microarray Accessories Cell Biology Apoptosis Antibodies Bone Research Products Cell Adhesion & Extracellular Matrix Cell Differentiation Kit Cell Proliferation & Viability Signal Transduction Viral & Microbial Detection Protein Research Recombinant Protein Folding & Expression C-Terminal Isolation & Analysis N-Terminal Deblocking & Analysis Protein Fragmentation Protease Inhibition Glycobiology Glycobiology Kits Related Enzymes Order Products
About Takara Bio USA Clontech-Takara Bio USA Merger Contact Us Takara Subsidiares and Distributors How to Order Career Opportunities
Press Releases Trade Shows Company News
Home > Products > Molecular Biology > Modifying Enzymes: Phosphatases > Alkaline Phosphatase, Calf Intestinal (CIAP), Cloned Details

Alkaline Phosphatase, Calf Intestinal (CIAP), Cloned

Applications:

  • Removing Phosphate Groups from 5'-ends of Linear Vectors to Prevent Recircularization during Cloning
  • Dephosphorylation of DNA prior to Kinase Labeling Reactions

Description:

Calf Intestinal Alkaline Phosphatase (CIAP) catalyzes the hydrolysis of 5'-phosphate groups from DNA and RNA. CIAP is supplied in 10 mM Tris-HCl (pH 8.0), 50 mM KCl, 1 mM MgCl2, 0.1 mM ZnCl2 and 50% glycerol

Source:

Yeast

Buffer:

Supplied with 10X Reaction Buffer [500 mM Tris-HCl (pH 9.0), 10 mM MgCl2].

Concentration:

10–30 U/μL

Unit Definition:

One unit is defined as the amount of enzyme that generates 1 μmol/min of p-nitrophenol from p-nitrophenylphosphate at 37°C and pH 9.8.

Storage:

–20°C

References:

  1. Sambrook, J. et al. (1989) Molecular Cloning: A Laboratory Manual, pp 5.72, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.

Alkaline Phosphatase, Calf Intestinal (CIAP), Cloned TAK 2250A 1000 U 78.00
Alkaline Phosphatase, Calf Intestinal (CIAP), Cloned TAK 2250B 5000 U (5 x 1000 U) 309.00
Product Name Product Number Size Price Qty Add

*All prices in USD