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Home > Products > Molecular Biology > Modifying Enzymes: Phosphatases > Alkaline Phosphatase, Shrimp Details

Alkaline Phosphatase, Shrimp

Applications:

  • Dephosphorylation of 5'-Terminal Phosphate Groups from DNA

Description:

Shrimp Alkaline Phosphatase (SAP) nonspecifically catalyzes the dephosphorylation of most phosphate monoesters, but it does not degrade diphosphate or triphosphate linkages. It is irreversibly inactivated by heat denaturation at 65°C for 15 minutes.

Source:

Shrimp (Pandalus borealis)

Buffer:

Supplied with 1ml 10X SAP Buffer [500 mM Tris-HCl (pH 9.0), 50 mM MgCl2].

Unit Definition:

One unit is the amount of enzyme that produces 1 μmol/min of p-nitrophenol per minute at 37°C and pH 9.6 using p-nitrophenylphosphate as the substrate.

Concentration:

1 U/μL

Storage:

–20°C

References:

  1. Rangnar, L. et al. (1991) Comp. Biochem. Physiol. 99B(4): 755-761.

Alkaline Phosphatase, Shrimp TAK 2660A 300 U 147.00
Alkaline Phosphatase, Shrimp TAK 2660B 1500 U (A x 5) 591.00
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