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T7 RNA Polymerase

Applications:

  • Synthesis of RNA Transcripts

Description:

T7 RNA Polymerase is a DNA-dependent RNA polymerase that exhibits a high specificity for bacteriophage T7 promoter sequences. The enzyme can incorporate labeled or unlabeled nucleotide triphosphates into an RNA transcript. Large quantities of RNA can be synthesized from a DNA sequence cloned downstream from a T7 promoter. T7 RNA Polymerase is supplied in 20 mM potassium phosphate (pH 7.9), 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA and 50% glycerol.

Unit Definition:

One unit is defined as the amount of enzyme that converts 1 nmol of [3H] GMP using a linear DNA template into acid-insoluble material in 1 hour at 37°C and pH 8.0.

Source:

Recombinant E. coli

Concentration:

10–50 U/μL

Buffer:

Supplied with 10X Reaction Buffer [400 mM Tris-HCl (pH 8.0), 80 mM MgCl2 and 20 mM spermidine], and 50 mM DTT.

Storage:

–20°C

T7 RNA Polymerase TAK 2540A 5000 U 56.00
T7 RNA Polymerase TAK 2540B (A x 5) 25,000 U 221.00
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