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T4 DNA Polymerase

Applications:

  • Creating Blunt Ends from Protruding Ends in Double-Stranded DNA Fragments
  • 3'-end Labeling of Double-Stranded DNA
  • Site-Directed Mutagenesis

Description:

T4 DNA Polymerase catalyzes the incorporation of nucleotides into double-stranded DNA in a 5'→3' direction. It possesses a strong 3'→5' exonuclease (proofreading) activity, but does not exhibit 5'→3' exonuclease activity. T4 DNA Polymerase is supplied in 200 mM potassium phosphate (pH 6.5), 10 mM DTT and 50% glycerol.

Source:

Recombinant E. coli

Buffer:

Supplied with 10X Reaction Buffer [330 mM Tris-acetate (pH 7.9), 660 mM potassium acetate, 100 mM magnesium acetate and 5 mM DTT] and 0.1% BSA.

Concentration:

2–5 U/μL

Unit Definition:

One unit is defined as the amount of enzyme that catalyzes the incorporation of 10 nmol of total nucleotides into acid-insoluble products in 30 minutes at 37°C and pH 8.8, using heat-denatured calf thymus DNA as the template-primer.

Storage:

–20°C

References:

  1. Kunkel, T.A.et al. (1987) Methods Enzymol. 154:367-82.
  2. Deen, K. C.et al. (1983) Anal. Biochem. 135:456-65.
  3. Wartell, R.M. and Reznikoff, W. S. (1980) Gene 9:307-19.

T4 DNA Polymerase TAK 2040A 100 U 79.00
T4 DNA Polymerase TAK 2040B (A x 5) 500 U 312.00
Product Name Product Number Size Price Qty Add

*All prices in USD