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Exonuclease III

Applications:

  • Preparation of Partially Single-Stranded DNA for Sequencing
  • Producing Nested Deletions in Double-Stranded DNA
  • Analyzing DNA-Protein Interactions

Description:

Exonuclease III is a 3'→5' exonuclease that is specific for double-stranded DNA or DNA-RNA hybrids. It will degrade double-stranded DNA with a blunt end, a 5'-overhang or a nick, but it will not degrade DNA with a 3'-overhang of at least 4 bases. Exonuclease III is supplied in 25 mM Tris-HCl (pH 8.0), 50 mM KCl, 0.5 mM DTT and 50% glycerol.

Source:

Recombinant E. coli

Buffer:

Supplied with 10X Reaction Buffer [500 mM Tris-HCl (pH 8.0), 50 mM MgCl2 and 100 mM DTT.]

Concentration:

100-300 U/μL

Unit Definition:

One unit is defined as the amount of enzyme that produces 1 nmol of acid-soluble products in 30 minutes at 37°C and pH 8.0, with restriction-digested calf thymus DNA as a substrate.

Storage:

–20°C (when stored for more than 6 months, it should be kept frozen at –80°C)

References:

  1. Wu, C. (1985) Nature 317:84-7.
  2. Guo, L.H. and Wu, R. (1983) Methods Enzymol. 100:60-96.

Exonuclease III TAK 2170A 5000 U 44.00
Exonuclease III TAK 2170B (A x 5) 25,000 U 176.00
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