One-Step RNA PCR Kit
Applications:
- Reverse transcription (RT) PCR from RNA templates in a single-tube, one-step reaction
Description:
Takara's One-Step RNA PCR Kit offers the convenience and time savings of performing reverse transcription and PCR in a single tube, without the need for changing buffers or adding reagents, minimizing the risk of contamination. AMV Reverse Transcriptase XL provides greater thermostability than other reverse transcriptases, and retains its activity over a wider range of reaction temperatures. The kit also contains AMV-Optimized Taq DNA Polymerase for efficient amplification of the cDNA synthesized by AMV Reverse Transcriptase XL.
Kit Components:
| TAK RR024A | |
| AMV Reverse Transcriptase XL* (5 U/μL) | 50 μL |
| AMV-Optimized Taq DNA Polymerase (5 U/μL) | 50 μL |
| RNase Inhibitor (40 U/μL) | 60 μL |
| RNase-Free dH2O (DEPC-treated) | 2 x 1 mL |
| 10X One-Step RNA PCR Buffer | 250 μL |
| dNTP Mixture (10 mM each) | 250 μL |
| MgCl2 (25 mM) | 500 μL |
| Control F-1 Primer (20 pmol/μL) | 25 μL |
| Control R-1 Primer (20 pmol/μL) | 25 μL |
| Positive control RNA (2 x 105 copies/μL) | 25 μL |
| *Manufactured by Life Science Co. | |
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