ApopLadder Ex™ (Apoptotic DNA Fragment Extraction Kit)
Applications:
- Sensitive, Specific and Rapid Method for the Isolation of Fragmented DNA
Description:
Apoptosis is associated with the fragmentation of chromosomal DNA into approximately 180 bp nucleosome fragments.
The ApopLadder Ex™ Kit selectively extracts the small, fragmented DNA from apoptotic cells and minimizes chromatin contamination. This method supports highly sensitive detection and quantitation of fragmented DNA. Detection of fragmented DNA following isolation can be observed through gel electrophoresis and can be quantitated by fluorescent staining with SYBR® Green I (Molecular Probes, Inc).
This kit contains the necessary reagents for cell lysis, enzymatic degradation of protein and RNA, precipitation of the DNA fragments, and a 6X loading dye for gel electrophoresis.
Kit Components:
| Lysis Buffer | 4 x 1.2 mL |
| 10% SDS | 1 x 0.48 mL |
| Enzyme A Solution | 1 x 0.48 mL |
| Enzyme B Solution | 1 x 0.48 mL |
| Precipitant | 3 x 1.04 mL |
| 6X Loading Dye | 1 x 0.48 mL |
Storage:
–20°C
