Procollagen Type I C-Peptide (PIP) EIA Kit (Precoated)
Applications:
- Quantitative Measurement of Human, Bovine, Dog or Horse PIP
Description:
Collagen types I, II, III, IV and V are synthesized as precursor molecules called procollagens. These precursor molecules contain additional peptide sequences, termed "propeptides", at both the amino-terminal and the carboxy-terminal ends. Propeptides function to facilitate the winding of procollagen molecules into a triple-helical conformation within the endoplasmic reticulum. The propeptides are cleaved from the collagen triple helix molecule during its secretion, after which the triple helix collagens polymerize into extracellular collagen fibrils. Thus, the amount of the free propeptides reflects stoichiometrically the amount of collagen molecules synthesized. In particular, pro-collagen type I carboxy-terminal peptide (PIP) has been used as a reference for studying the correlation of collagen levels with certain health disorders, including bone diseases, alcoholic liver diseases, liver cirrhosis, and scirrhous (Borrmann type IV) adenocarcinoma of the stomach.
The Procollagen Type I C-Peptide EIA Kit is a 96 well in vitro enzyme immunoassay for the quantitative determination of human, bovine, or canine PIP in plasma, serum, cultured cell extracts, cell culture supernatants, and other biological fluids.It is a solid phase EIA based upon a sandwich design that utilizes two mouse monoclonal PIP antibodies (one of which is coated on the plate, and the other is POD-labeled) for detection of PIP using a one step incubation method. Samples and POD-anti-PIP are simultaneously added to the wells of the plate and incubated. During incubation, PIP becomes bound to the antibody coating the plate, and is then tagged by POD-anti-PIP on its opposite side. A substrate is added, and the reaction between POD and the substrate (H2O2, TMBZ) results in a color development. The amount of sample PIP is determined by measuring absorbance using an EIA plate reader. Accurate PIP sample concentrations can be determined by comparing their specific absorbances with the absorbance obtained for the Standards plotted on a standard curve.
Kit Components:
| Antibody Coated Microtiter Plate | 1 plate (8 well x 12 strips) |
| Antibody-POD Conjugate (lyophilized) | 1 vial (for 1 x 11 mL) |
| Standard (lyophilized) | 1 vial (for 1 x 1 mL) |
| Sample Diluent | 2 vials (2 x 11 mL) |
| Substrate Solution | 1 vial (1 x 12 mL) |
Performance Characteristics
| Range of assay: | 10-640 ng/mL |
| Assay sensitivity: | 10 ng/mL |
| Assay duration: | 3.5 hours |
| Specificity: | Human PIP. No reactivity with fibronectin, vitronectin, laminin, collagen type I, or collagen type III. |
| Species cross-reactivity: | Reactivity with bovine, dog and horse PIP. No reactivity with mouse PIP. Applications with other sources have not been tested. |
| Test specimen type: | Serum, plasma, culture supernatants (serum-free), and cell extracts |
| Sample volume per well: | 20 μL |
Storage:
2–8°C
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