TRACP & ALP Assay Kit
Applications:
- Detection of Acid Phosphatase (ACP) and Alkaline Phosphatase (ALP)
Description:
TRACP & ALP Assay Kit has been designed for simple and quick detection of ACP (Acid phosphatase) and ALP (Alkaline phosphatase) by utilizing pNPP (p-nitro-phenyl phosphate) substrate. This kit achieves the simultaneous detection of 2 enzymes which are involved in bone metabolism, TRACP and ALP which is an enzyme marker of osteoblasts. The addition of tartaric acid into the ACP assay allows for the detection of TRACP (Tartrate-Resistant Acid Phosphatase) activity and enzyme marker of osteoclast. Since this kit utilizes an aqueous substrate, it allows for quick quantification of the enzyme activity by measuring the absorbance of the reactant. In addition to this kit, a TRACP & ALP Double-Staining Kit (TAK MK300) is also available for the assay using a non-soluble substrate. The appropriate kit can be selected depending on an assay of interest.
Kit Components:
| pNPP (p-nitro-phenyl phosphate) substrate [pNPP substrate] | 5 vials x 24 mg |
| Extraction solution | 2 vials x 11 mL |
| Sodium tartrate solution | 4 mL |
| Buffer for ACP | 30 mL |
| Buffer for ALP | 30 mL |
| Microplate (96-well)* | 1 plate |
| *The plate is reusable after soaking in 1% sodium hypochlorite solution overnight. | |
Reagent required but not supplied in the kit:
| Stop Solution* | 0.9 N NaOH* |
| *Prior to starting the assay, Stop Solution must be prepared. Please note this solution is corrosive and may cause inflammation when it comes in contact with skin. If it should come in contact with hands or mucous membranes, immediately wash with large amounts of water and follow instructions provided by doctors. | |
Storage:
4°C
