RACE Core Set, 3'-Full
Applications:
- Rapid amplification of cDNA ends, cloning full-length cDNA sequences
Description:
Reverse transcription of mRNA followed by PCR is a common method for obtaining cDNA clones for expression analysis. However, it can be difficult to obtain full-length cDNA by this method. Rapid amplification of cDNA ends (RACE) helps overcome this problem by using an oligo dT primer for reverse transcription, followed by amplification with specially designed oligonucleotide primers.
The 3'-Full RACE Core Set uses a specially designed Oligo dT Adaptor Primer for efficient synthesis from the 3'-end of poly(A) RNA. The Adaptor Primer has 3 restriction sites for easy cloning after RT-PCR. Subsequent amplification uses this adaptor-primer in conjunction with a specific primer for the gene of interest.
The kit uses AMV Reverse Transcriptase XL to maximize the length of the first-strand cDNA, and is recommended for use with Takara's Taq™, Ex Taq™ or LA Taq™.
Kit Components:
| TAK 6121 | |
| AMV Reverse Transcriptase XL* (5 U/μL) | 20 μL |
| RNase Inhibitor (40 U/μL) | 10 μL |
| 10X RNA PCR Buffer | 40 μL |
| dNTP Mixture (10 mM each) | 40 μL |
| MgCl2 (25 mM) | 80 μL |
| RNase-Free dH2O (DEPC-treated) | 500 μL |
| Oligo dT-3 Sites Adaptor-Primer (2.5 μM) | 20 μL |
| 3 Sites Adaptor Primer (20 μM) | 20 μL |
| Control F-1 3 Sites Adaptor Primer (20 μM) | 10 μL |
| Positive Control RNA (2 x 105 copies/μL) | 10 μL |
| *Manufactured by Life Science Co. | |
Storage:
–20°C
