O-157 (Verocytotoxin Genes) One-Shot PCR Typing Kit
Applications:
- Simple detection of both type 1 and 2 of the Verocytotoxin genes from EHEC
Description:
This kit enables simple detection of Verocytotoxin genes from EHEC while simultaneously distinguishing between type 1 and type 2 Verocytotoxin genes in a single tube. The premixed solution (2X One Shot PCR solution in 0.2 mL tubes) contains all the reagents required to perform PCR including specific primers and TaKaRa Ex Taq™. Detection can be initiated by simply adding a sample template DNA.
Because this kit uses high efficiency TaKaRa Ex Taq™, highly sensitive detection is possible in a shorter time than using regular Taq. Furthermore, positive control template is supplied in each tube of this kit to prevent false negative detection. Because the size of the fragments amplified from this template (1070 bp) is easily distinguished from those obtained from Verocytotoxin gene, the existence of Verocytotoxin gene can be easily verified by gel electrophoresis.
Kit Components:
| TAK RR106A | |
| 2X One-Shot PCR solution | 48 x 50 μL |
| (supplied in 0.2 mL PCR tube) | |
Storage:
–20°C. Avoid repeated freeze-thaws because each tube contains TaKaRa Ex Taq™.
Limited Use Label License: [A1][M57]
