α1,3/4-L-Fucosidase
Substrate specificity:
- Specifically cleaves α1,3 and α1,4 fucoside bonds. Liberates L-fucose from lacto-N-fucopentaose II, lacto-N-fucopentaose III and N-acetyllactosamine type tri-antennary and tetra-antennary sugar chain with α1,3 fucoside bonds.
- Also liberates L-fucose from α1-acid glycoprotein.
Systematic Name:
α-L-Fucoside fucohydrolase (E.C. 3.2.1.51)
Source:
Streptomyces sp. 142
Form:
Solution in 50 mM potassium phosphate buffer (pH 6.0) containing 0.1 M NaCI, 0.02% NaN3, and 0.1% Brij-58
Concentration:
1 mU/mL
Properties:
| Molecular weight: | 40 kDa |
| Km (substrate): | Substrate 1: 10.1 μM (PA-lacto-N-fucopentaose II) |
| Substrate 2: 1.08 μM (PA-lacto-N-fucopentaose III) | |
| Optimum pH: | pH 6.0 (Substrate 2) |
| Range of pH stability: | pH 4.5–7.0 (37°C, 60 min.) |
| Stabilizer: | 0.1% NP-40, 0.1% Brij-58, and 0.02% BSA |
Definition of Activity:
One unit is the amount of enzyme required to hydrolyze 1 μmol of pyridylamino-lacto-N-fucopentaose III within 1 minute at 37°C at pH 6.0.
Storage:
Store at –20°C. The stock solution is stable for at least 12 months at 4°C. Avoid repeated freeze-thaw cycles.
References:
- Sano, M. et al. (1992) J. Biol. Chem. 267:1522-7.
