SCDase (Sphingolipid ceramide N-deacylase)
Applications:
- For hydrolysis of N-acyl linkage between fatty acids and sphingosine bases
Description:
SCDase (Sphingolipid ceramide N-deacylase) from Pseudomonas species hydrolyzes the N-acyl linkage between fatty acids and sphingosine bases in ceramides of various sphingolipids. The enzyme also catalyzes the reverse reaction, and possesses transacylation activity. The enzyme acts on various acidic and neutral glycosphingolipids and sphingomyelin; however, it exhibits low activity on ceramide.
Source:
Pseudomonas sp.
Concentration:
5 mU/μL
Form:
Solution in 50 mM sodium acetate (pH 6.0) containing 0.1% Lubrol PX
Properties:
| Inhibitors: | Hg2+, Zn2+ and Cu2+ |
| Not inhibited by: | MN2+, Ca2+, Mg2+ or EDTA |
| Optimum pH: | Forward reaction pH 5.0–6.0 (0.8% TRITON® X-100); Reverse reaction pH 7.0 (0.1% TRITON® X-100) |
Definition of Activity:
One unit is the amount of enzyme required to catalyze the hydrolysis of 1 μmol of asialo GM1 per minute.
Storage:
Store at -20°C until use. Store reconstituted solution in aliquots at -20°C. Avoid freeze-thaws.
References:
- Mitsutake, S.et al. (1998) J. Biochem. (Japan) 123:859-63.
- Tani, M. et al. (1998) Anal. Biochem. 263:183-8.
- Mitsutake, S. et al. (1997) Anal. Biochem. 247:52-7.
- Sueyochi, N. et al. (1997) J. Lipid Res. 38:19233-27.
- Ito, M. et al. (1995) J. Biol. Chem. 270:24370-4.
