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Home > Products > Glycobiology > Related Enzymes > Lacto-N-Biosidase Details

Lacto-N-Biosidase

Substrate specificity:

  • This enzyme specifically hydrolyzes oligosaccharides with type 1 chains and produces lacto-N-biose (Galβ-3GlcNAc).
  • The enzyme does not hydrolyze oligosaccharides with type II chain.
  • The structure of the substrate essential for the enzyme activity is the terminal lacto-N-biosyl residue (Galβ1-3GlcNAc).
  • The enzyme does not hydrolyze α2,3-sialyllacto-N-tetraose, lacto-N-fucopentaose I and II.

Source:

Streptomyces sp. 142

Concentration:

1 µU/µL

Form:

Solution in 50 mM sodium acetate buffer, pH 5.5, containing 0.05% Brij 58

Properties:

Molecular weight: 60 kDa (SDS-PAGE)
Km (substrate): 6.80 μM Pyridylamino (PA)-lacto-N-tetraose); 38.9 μM (PA-oligosaccharide c)
Optimum pH: pH 5.5 (sodium acetate buffer)
Range of pH stability: pH 4.0–10 (4°C, 16 hrs.)

Definition of Activity:

One unit is the amount of enzyme required to hydrolyze 1 μmol of PA-lacto-N-tetraose in 1 minute at 37°C, pH 5.5.

Storage:

The enzyme solution of 1 mU/mL is stable for at least 12 months at below 4°C.

References:

  1. Sano, M. et al. (1992) Proc. Natl. Acad. Sci. USA 89:8512-6.

Limited Use Label License: [M15][M16]

Lacto-N-Biosidase TAK 4456 100 μU 485.00
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