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Chaperone Plasmid Set

Applications:

  • Correct in vivo folding of expressed recombinant proteins in E. coli

Description:

The Chaperone Plasmid Set consists of 5 different plasmids, each of which is designed to express multiple molecular chaperones that function as a "chaperone team" to enable protein folding. Co-expression of a target protein with one of these chaperone teams increases the recovery of soluble proteins. Each plasmid carries an origin of replication derived from pACYC and a Cmr gene, which allows use with E. coli expression systems utilizing ColE1-type plasmids containing an ampicillin resistance gene as a marker. The chaperone genes are situated downstream of an araB orPzt-1 (tet) promoter, thus, expression of target proteins and chaperones can be induced individually if the target gene is placed under the control of other promoters (e.g. lac). These plasmids also contain the necessary regulator (araC or tetr) for each promoter.

Compatible E. coli expression systems
  1. These chaperone plasmids carry the pACYC origin of replication and a chloramphenicol-resistance gene (Cmr gene); this allows use with stranded E. coli expression systems utilizing colE1-type plasmids with ampicillin resistance gene as a marker. This system cannot be used in combination with chloramphenicol-resistant E. coli host strains or expression plasmids that carry a chloramphenicol-resistance gene. For example, E. coli BL21(DE3), which is often used with pET systems, etc., is acceptable as a host strain, but E. coli BL21(DE3) pLysS and BL21(DE3) pLysE, which contains the  pLysS or pLysE plasmids that have the pACYC replication origin and the Cmr gene, cannot be used with this system.
  2. This product prescribes a two-step method to construct target/chaperone co-expression systems.
  3. The 1st step is to prepare an E. coli host transformed with only the chaperone plasmid. The 2nd step is to prepare competient cells from this new strain and to transform this strain with a plasmid expressing the target protein.

This system works well with the pCold Expression System Vectors.

Kit Components:

Plasmid pG-KJE8 (10 ng/µL) 100 µL
Plasmid pGro7(10 ng/µL) 100 µL
Plasmid pKJE7(10 ng/µL) 100 µL
Plasmid pGTf2(10 ng/µL) 100 µL
Plasmid pTf16(10 ng/µL) 100 µL

References:

  1. Nishihara, K. et al. (2000) Microbiol. 66:884-889.
  2. Nishihara, K., et al. (1998) Appl. Environ. Microbiol. 64:1694-1699.

Notes:

The intellectual property of the plasmids supplied in this product is owned by TAKARA BIO INC. This product is intended for research purposes only. Use of this product for commerical purposes such as screening or production requires a separate commercial contract with TAKARA BIO INC. The sequence information on the plasmids supplied in this set are not disclosed, and it is prohibited to modify this product or to use this product for plasmid preparation without prior permission from TAKARA BIO INC.

Figure Thumbnails:

Chaperone Plasmid Set TAK 3340 1 set 221.00
Product Name Product Number Size Price Qty Add

*All prices in USD