Bacillus anthracis PCR Detection Kit
Applications:
- Detection of Bacillus anthracis using PCR
Description:
The virulence of Anthrax (Bacillus anthracis) is due to the presence of two plasmids (pX01 and pX02). Plasmid X01 encodes three toxin components (PA: protective antigen, LF: lethal factor, EF: edema factor). Plasmid pX02 encodes a group of genes involved in capsule synthesis (capA, capB, and capC). Both of these plasmids must be present in the organism for virulence.
This kit is designed to detect the PA gene contained in plasmid pX01 and the capA gene in plasmid pX02 via intercalation real-time PCR.
This kit includes Takara's efficient Hot-Start PCR enzyme, TaKaRa Ex Taq™ HS, which results in very sensitive detection and reduced non-specific amplification due to mispriming or primer-dimers. The kit also contains an internal control for monitoring false negatives.
Kit Components:
| TAK RR027 | |
| TaKaRa Ex Taq™ HS Polymerase (5 U/μL) | 12.5 μL |
| 5X Reaction Mixture* | 500 μL (96 reactions) |
| PA Primers (PA7, PA6) (10 μM each) | 200 μL |
| CAP Primers (M011, M012) (10 μM each) | 200 μL |
| 100 bp DNA Ladder (650 ng/5 μL) | 50 μL |
| 6X Loading Buffer** | 60 μL |
| *includes dNTP Mixture and Internal Controls. | |
| **36% Glycerol, 30 mM EDTA, 0.06% Bromophenol Blue, 0.06% Xylene cyanol. | |
| NOTE: This kit is designed for a quick, preliminary result. For definitive assessment, this test should be combined with a general microbial test, such as gram staining. | |
