Carboxypeptidase P
Applications:
- C-terminal Sequence Determination
Description:
Carboxypeptidase P non-specifically removes amino acids, including proline, from the carboxy termini of proteins.
Source:
Penicillium janthinellum
Definition of Activity:
One unit of enzyme activity corresponds to the amount required for hydrolysis 1 μmol of Cbz-Glu-Tyr in 1 minute at pH 3.7, 30°C.
Form:
Lyophilized, containing sodium citrate as a stabilizer
Purity:
No other proteases detected
Properties:
| Molecular weight: | 51 kDa (gel filtration) |
| Optimum pH: | 3.7 (Cbz-Glu-Try) |
| 4.5* (Cbz-Gly-Pro-Leu-Gly) | |
| 5.2 (Cbz-Gly-Lyz) | |
| Inhibitors:** | Monoiodoacetic acid (MIA); disopropylfluorophosphate (DFP); p-chloromercuribenzoate (PCMB) |
| pH stability: | 20 min. at pH 3.0-7.0, 30°C |
| Thermal stability: | 10 min. at pH 3.1, 40°C |
| 10 min. at pH 3.7, 50°C | |
| *Glycine is rapidly released; a small amount of leucine is released at pH 5.2 under higher concentrations and prolonged incubation. | |
| **EDTA and o-phenanthroline do not affect activity. | |
Storage:
4°C (under dry conditions)
