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Home > Products > Protein Research > N-Terminal Deblocking & Analysis > Pfu Pyroglutamate Aminopeptidase Details

Pfu Pyroglutamate Aminopeptidase

Applications:

  • Removal of Pyroglutamic Acids from the N-termini of Proteins and Peptides
  • Deblocking of N-terminal Pyroglutamates of Proteins and Peptides for Sequence Analysis using Edman Degradation

Description:

Pfu Pyroglutamate Aminopeptidase liberates the N-terminal pyroglutamic acid from proteins and peptides. This enzyme may work well with some intact, non-denatured proteins and, thus, the denaturation step may be unnecessary in these instances. This product is supplied with 5X Reaction Buffer [250 mM sodium phosphate (pH 7.0), 50 mM DTT, 5mM EDTA].

Systematic Name: L-Pyrrolidone carboxyl peptidase. Enzyme code: 3.4.19.3

Properties:

Molecular weight: 24.072 kDa (calculated)
28 kDa (by SDS-PAGE)
Optimum temperature: 95–100°C
Thermal stability: approx. 90% activity at 75°C
+ pH 7.0, 150 min.
Optimum pH: 6.0–9.0
Stable pH range: 5.0–9.0
≥80% activity in this range 5.0-9.0 at 75°C when reactived for 30 min.
Tolerance to denaturants: ≤1 M Urea
≤1 M Guanidine-HCl
≤0.01% SDS
Inhibitors: PCMB, Hg2+

 

Supplied Buffer (5X)
Volume: 1 mL
Component: 250 mM sodium phosphate buffer (pH 7.0) containing 50 mM DTT and 5 mM EDTA
Comparison of specific activities at various temperatures (an example)
Enzyme with a specific activity of 5.83 U/mg protein at 37°C showed the activities of 12.2 U/mg protein or 28.3 U/mg protein at 50°C or 75°C, respectively.

Source:

Escherichia coli carrying plasmids encoding the Pyrococcus furiosus pyroglutamate aminopeptidase gene.

Form:

Lyophilized

Purity:

≥90% on SDS-PAGE. No other proteases detected.

Definition of Activity:

One unit of enzyme activity corresponds to the amount required to hydrolyze 1 μmol pyroglutamate p-nitroanilide at 37°C in 1 minute, pH 7.0.

References:

  1. Shimada, Y. et al. (1989) J. Biochem. 106:383.
  2. Hamazume, Y. et al. (1987) J. Biochem, 101:217.

 

Limited Use Label License: [L10][M33]]

Figure Thumbnails:

Pfu Pyroglutamate Aminopeptidase TAK 7334 10 mU 243.00
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