Pfu Protease S
Applications:
- Fragmentation of Proteins and Peptides required for Primary Structure Analysis
Description:
Pfu Protease S is an endo-type serine protease with broad specificity for native and denatured proteins. Cleavage occurs mainly on the carboxy side of peptide bonds of hydrophobic amino acid residues.
Source:
Bacillus species carrying a plasmid that encodes the Pyrococcus furiosus protease gene
Form:
Solution in 25 mM Tris-HCl (pH 7.6) containing 40% ethanol
Purity:
Homogeneous on SDS-PAGE. No other proteases detected.
Properties:
| Molecular weight: | 45 kDa (SDS-PAGE) |
| 42.906 kDa (calculated) | |
| Optimum temperature: | 85–95°C |
| Optimum pH: | 6.0–8.0 |
| Inhibitor: | PMSF |
| Tolerance to denaturants at 95°C pH 7.0: | |
| 1% SDS; retains 50% activity after 24 hrs. at 95°C, pH 7.0 | |
| 6.4 M urea; retains 70% activity after 1 hr. at 95°C, pH 7.0 | |
| 50% acetonitrile; retains 90% activity after 1 hr. at 95°C, pH 7.0 | |
Definition of Activity:
One unit of enzyme activity corresponds to the amount required to hydrolyze 1 mmol of N-succinyl Ala-Ala-Pro-Phe p-nitroanilide in 1 minute at 95°C, pH 7.0.
Limited Use Label License: [L10][M36]
