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Home > Products > Cell Biology > Antibodies > TaKaRa Taq Polymerase, Monoclonal Antibody Details

TaKaRa Taq Polymerase, Monoclonal Antibody

Applications:

For more information, see the Antibody Applications Summary.

Description:

This product is a monoclonal antibody to Taq DNA polymerase for use in Hot Start PCR. By binding to Taq DNA polymerase, this antibody inhibits the polymerase activity and prevents non-specific amplification derived from mispriming and/or formation of primer dimer before PCR cycling. The antibody is denatured during the initial denaturation step, so there is no need for a special denaturation step. The general PCR conditions can be adopted. Takara has confirmed that Taq antibody works effectively in conjunction use with TaKaRa Taq™ (TAK R001), TaKaRa Ex Taq™ (TAK  RR001), TaKaRa LA Taq™ (TAK RR002), and TaKaRa Z-Taq™ (TAK  R006).

Performance Test. Inhibition of Taq DNA polymerase activity by the antibody is confirmed to be more than 90% in the reaction at 55ºC for 10 min. after the incubation of the mixture of Taq antibody and Taq DNA polymerase at 25ºC for 10 min.
Taq DNA polymerase activity is confirmed to recover almost 100% in the reaction of 55ºC for 10 min. after heat treatment at 94ºC for 30 sec.

Concentration:

5 units/µL

Form:

22 mM Tris-HCl (pH8.0), 100 mM KCl, 15 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 0.5 % Tween 20, 0.5 % Nonidet P-40, 50 % Glycerol

Purity:

  • Nicking activity, endonuclease and exonuclease activity are not detected after the incubation of 1 µg of supercoiled pBR322 DNA, 1 µg of λDNA or 1 µg of λ-Hind III digest with 10 units of Taq antibody for 1 hour at 37ºC and 70ºC.
  • RNase activity is not detected after incubation of 1 µg of 16S, 23S rRNA with 10 units of Taq antibody for 1 hour at 37ºC.
  • No contamination with mouse genomic DNA is confirmed by 35 cycles of PCR.

Notes:

For research use only. Not for use in diagnostic or therapeutic procedures.

Storage:

–20°C

TaKaRa Taq Polymerase, Monoclonal Antibody TAK 9002A 250 U 108.00
TaKaRa Taq Polymerase, Monoclonal Antibody TAK 9002B 1000 U 347.00
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